|NPH/Isophane by Eli Lilly|
|Action in dogs:|
|Also known as: Humulin N, Humulin I, Humulin NPH, Humulina NPH, Humuline N, Humuline NPH, Umuline NPH Humulin ReliOn N|
| Similar to: Novolin N, Insulatard, Protaphan|
Insuhuman Basal, Protaphane, Winthrop Basal
ReliOn/Novolin NPH, Insuman Basal
Names of Lilly r-DNA/GE/GM insulins worldwide
|Use and Handling:|
|Shelf Life: 24 months||Type: cloudy|
|When Opened: 28 days room temp.|
|In Pen: 28 days room temp.|
| Notes: Protect from light and heat|
Do Not Freeze, Resuspend
Do not use if product does not re-suspend
Do not use intravenously 
Note that some countries have the brand name in U40 strength.
It is an intermediate-acting insulin.
NPH (Isophane) insulin was originally developed by Novo Nordisk labs in 1946, as an insulin with intermediate action.
This type of insulin tends to have an onset of 2-4 hours, a peak action of 4-10 hours, and a total duration of 10-20 hours, in humans.
In the UK, Humulin N is known as Humulin I ("I" standing for its isophane suspension.);  in the US, it's called Humulin N. Elsewhere it's called Umuline NPH, Humulina NPH and Humuline NPH. Aventis also makes two similar NPH insulins called Insuman Basal  and Winthrop Basal.
Specifications of NPH/isophane insulinEdit
British National Formulary  defines this type as: A sterile suspension bovine or porcine or of human insulin in the form of a complex obtained by the addition of protamine sulphate or another suitable protamine.
British Pharmacoepia and United States Pharmacopia definitions: 
Sterile buffered suspension of insulin in the form of a complex obtained by addition of suitable protamine. Prepared from crystalline insulin. pH 6.9 - 7.5 iso-osmotic with blood. Contains for each 100 units of insulin, 300 - 600 g protamine sulphate and not more than 40 g zinc, a suitable bactericide and sodium phosphate as buffering agent.
USP specification: Sterile suspension of zinc insulin crystalline and protamine sulphate in buffered water for injection. Solid phase contain crystals of insulin protamine and zinc; 40, 80, 100 units/ml. Contains glycerol, metacresol, phenol sodium phosphate and zinc.
The following commercial insulins are all generically NPH insulins:
|All Intermediate acting|
|Betasint Bovine NPH|
Hypurin Bovine Isophane 
(No longer produced.)
|Iletin I NPH
(No longer produced.)
|Betasint Porcine NPH|
Hypurin Porcine Isophane 
Hypurin Pork NPH
|Iletin II NPH (No longer produced.)|
Pork Insulatard 
(No longer produced.)
Leo Retard (No longer produced.)
|Huminsulin Basal, Humulin I|
Humulin N, Humulin NPH
Humulina NPH, Humuline N
Humuline NPH, Umuline NPH
Humulin ReliOn N
|Insuhuman Basal, Insuman Basal|
Isuhuman Basal, Winthrop Basal
|Insulatard, Novolin N|
(No longer produced)
Other commonly used names are:
- NPH insulin, NPH
- isophane insulin
- ↑ Insulins. North American Veterinary Conference (2005).
- ↑ Ruchinsky, Renee, et. al. (2010). Diabetes Management Guidelines for Dogs and Cats-page 7. American Animal Hospital Association.
- ↑ Maddison, Jill E.,Page, Stephen W.,Church, David B. (2008). Small Animal Clinical Pharmacology. Saunders Ltd..
- ↑ Humulin N NPH/Isophane Human Insulin. Revolution Health.
- ↑ Humulin N patient leaflet. Eli Lilly.
- ↑ Novolin N. Revolution Health.
- ↑ Patient Information Leaflet-Insulatard. Novo Nordisk.
- ↑ 8.0 8.1 Prescribing Novo Insulins.
- ↑ Scientific Discussion-Insulatard. EMEA.
- ↑ Scientific Discussion-Protaphane. EMEA.
- ↑ Humulin I Prescribing Information.
- ↑ Patient Information Leaflet-Humulin I. Eli Lilly.
- ↑ Lilly EU/UK Product Sheet & Time Activity Profiles. Eli Lilly.
- ↑ Patient Information Leaflet-Insuman Basal. Sanofi-Aventis.
- ↑ Insuman Prescribing Information.
- ↑ 16.0 16.1 British National Formulary-Isophane/NPH Insulin Definition. InChem.
- ↑ Hypurin Bovine Isophane Patient Information. Net Doctor uk..
- ↑ Hypurin Bovine Isophane. Wockhardt UK.
- ↑ Prescribing Bovine Insulin.
- ↑ 20.0 20.1 Response to Withdrawal of Novo Porcine Insulins. CP/Wockhardt UK.
- ↑ Prescribing Porcine Insulin.
- ↑ Patient Information Leaflet-Pork Insulatard. Novo Nordisk.
- NACDS-Insulin Chart-Page 2
- Insulin therapy for dogs and cats Dowling, Patricia, September 1995, Canadian Veterinary Journal
A discussion of NPH/isophane insulins.